Aquarium glossary

Biological filtration

BioFilter
DefinitionBiological filtration is the bacterial process that converts toxic ammonia to nitrite to nitrate via Nitrosomonas and Nitrospira colonies on filter media, substrate, and surfaces.

In depth

Biological filtration is the foundation of aquarium chemistry - the nitrogen cycle in action. Two distinct bacteria genera handle the work: Nitrosomonas oxidizes ammonia (NH3) to nitrite (NO2). Nitrospira oxidizes nitrite to nitrate (NO3). These colonies live primarily on biological media (ceramic rings, bio-balls, K1, sintered glass) and live rock. Surface area drives capacity - that's why "1 liter of bio-media per 100 liters of water" is the rule of thumb. Bacteria die from: chlorine, antibiotics that target gram-negatives (Maracyn 2 less so), 24h+ without ammonia source, drying out. Bacteria thrive on: consistent ammonia source, steady oxygen, neutral pH, 75-82°F.

Reviewed by the Fast Aquatics husbandry team · Updated May 2026

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